HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ,Ⅱ的含量

陈清; 夏亚飞; 阎姝

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HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ,Ⅱ的含量

更新时间：2024-01-04

- HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ,Ⅱ的含量
- Comparative on Content of Paridis SaponinsⅠ,Ⅱ between Ethanol-Extraction and Granule of Rhizoma Paridis by HPLC
- 中国实验方剂学杂志 2012年18卷第6期页码：78-80
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  天津市科委项目(10JCYBJC15100)
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- 纸质出版日期：2012
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陈清, 夏亚飞, 阎姝. HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ,Ⅱ的含量[J]. 中国实验方剂学杂志, 2012,18(6):78-80.

CHEN Qing, XIA Ya-fei, YAN Shu. Comparative on Content of Paridis SaponinsⅠ,Ⅱ between Ethanol-Extraction and Granule of Rhizoma Paridis by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 78-80.
陈清, 夏亚飞, 阎姝. HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ,Ⅱ的含量[J]. 中国实验方剂学杂志, 2012,18(6):78-80. DOI：

CHEN Qing, XIA Ya-fei, YAN Shu. Comparative on Content of Paridis SaponinsⅠ,Ⅱ between Ethanol-Extraction and Granule of Rhizoma Paridis by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 78-80. DOI：

摘要

目的: 建立一种测定重楼皂苷Ⅰ

Ⅱ的含量测定方法

用于比较重楼乙醇提物与重楼配方颗粒中皂苷的含量。方法: 采用C18色谱柱

以乙腈-水(42:58)为流动相

检测波长210 nm

流速1 mL·min-1

柱温室温。结果: 在0.043 5~0.870 0 g·L-1

重楼皂苷Ⅰ的峰面积与浓度有良好的线性

r=0.999 1;在0.038~0.760 0 g·L-1

重楼皂苷Ⅱ的峰面积与浓度有良好的线性

r=0.999 7

重楼皂苷Ⅰ

Ⅱ的平均回收率为99.90%

100.2%

RSD分别为1.7%

1.7%。醇提物与配方颗粒中皂苷Ⅰ

Ⅱ的总含量分别为34.7%

3.1%。结论: 该法操作简单

出峰时间短

两峰分离度好

测得的醇提物中皂苷Ⅰ

Ⅱ含量明显高于重楼配方颗粒。

Abstract

Objective: To establish a determination method of the paridis saponinsⅠand Ⅱ for the content comparison of ethanol-extraction and granule of paridis. Method: Kromasil C18column(4.6 mm×250 mm

5 μm)was used.Acetonitrile-water(42: 58)was used as a mobile phase.Flow rate was 1 mL·min-1.Detection wavelength was at 210 nm

column temperature was in room temperature. Result: The Paridis saponinsⅠconcentration and peak area was showed good linear relationship between 0.0435-0.87 g·L-1(r=0.999 1).Paridis saponins Ⅱ concentration and peak area was showed good linear relationship between 0.038-0.76 g·L-1(r=0.999 7).recovery of Paridis saponinsⅠand saponins Ⅱ was 99.90% and 100.2% separately.RSD is 1.6% and 1.34%

the content of paridis saponin Ⅰand Ⅱ in ethanol-extraction and granule is 34.7% and 3.1% respectively. Conclusion: This method is simple with short ratention time and well separation

the content of saponin Ⅰand Ⅱ in ethanol-extraction were significantly higher than that of granule.

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