HPLC测定截叶铁扫帚不同药用部位中槲皮素、山奈酚的含量

朱晓勤; 彭水梅; 吴锦忠

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HPLC测定截叶铁扫帚不同药用部位中槲皮素、山奈酚的含量

更新时间：2024-01-04

- HPLC测定截叶铁扫帚不同药用部位中槲皮素、山奈酚的含量
- Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of by HPLC
- 中国实验方剂学杂志 2012年18卷第10期页码：80-83
- 作者机构：
- 作者简介：
- 基金信息：
  
  福建中医学院服务海西建设重点项目;福建省教育厅科技项目(JA10167)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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朱晓勤, 彭水梅, 吴锦忠. HPLC测定截叶铁扫帚不同药用部位中槲皮素、山奈酚的含量[J]. 中国实验方剂学杂志, 2012,18(10):80-83.

ZHU Xiao-qin, PENG Shui-mei, WU Jin-zhong. Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 80-83.
朱晓勤, 彭水梅, 吴锦忠. HPLC测定截叶铁扫帚不同药用部位中槲皮素、山奈酚的含量[J]. 中国实验方剂学杂志, 2012,18(10):80-83. DOI：

ZHU Xiao-qin, PENG Shui-mei, WU Jin-zhong. Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 80-83. DOI：

摘要

目的: 建立高效液相色谱法同时测定截叶铁扫帚不同药用部位中槲皮素、山奈酚含量的方法。方法: 采用Daisogel Sp-ODS-BP C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.2%磷酸(63∶37)

流速1.0 mL·min-1

柱温25 ℃

检测波长360 nm。结果: 槲皮素在6.25~100 mg·L-1呈良好的线性关系(r=0.999 9)

加样回收率100.75%

RSD 1.92%

山奈酚在0.638~20.4 mg·L-1呈良好的线性关系(r=0.999 9)

加样回收率为99.76%

RSD 1.99%。根、枝、叶部位槲皮素的平均质量分数分别为9.00

41.79

221.86 μg·g-1

山奈酚的平均质量分数分别为3.09

7.52

40.72 μg·g-1。结论: 该方法简便快速

结果准确可靠

可作为截叶铁扫帚不同药用部位的含量测定方法

为合理开发利用截叶铁扫帚资源提供理论依据。

Abstract

Objective: To develop a HPLC method for the determination of the content of quercetin and Kaempferol from different medicinal parts of Lespedeza cuneata. Method: Samples were analyzed on Daisogel Sp-ODS-BP C18(4.6 mm×250 mm

5 μm)

with the mobile phase consisted of methanol-0.20% phosphoric acid solution (63∶37). The flow rate was 1.0 mL·min- 1

column tomparature was at 25 ℃. The UV wavelength was set at 360 nm. Result: The linear range of quercetin was 6.25-100 mg·L-1. The average recovery was 100.75% and RSD was 1.92%). The linear range of kaempferol was 0.638-20.4 mg·L-1.The average recovery was 99.76% and RSD was 1.99%. The concent of quercetin in root

branch and leaf was 9.00

41.79

221.86 μg·g-1. The concent of kaempferol was 3.09

7.52

40.72 μg·g-1. Conclusion: The method is simple

rapid

accurate and reliable. It can be used as a content determination method for the medicinal parts of L. cuneata. It can provide evidence for the rational development and utilization of L. cuneata.

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