Objective: To establish a rapid

accurate and standardized method for identifying Gentianaceae mongolian medicine Digeda with DNA barcoding method

and to provide the basis of molecular authentication of mongolian medicine Digeda. Method: The mongolian medicine Digeda was amplified by PCR with universal primer and sequenced directly

the sequence was aligned and analyzed. Result: The complete rbcL

ITS

trnH-psbA and matK sequence of four Digeda species have been obtained

and the length of four sequences region varied from 388 to 940 bp. They were distinguished by rbcL

ITS or matK sequence. Conclusion: The trnH-psbA sequence was not suitable to identify the four species of Digeda. The ITS region could be a pretty good marker to distinguish mongolian medicine Digeda. DNA barcoding identification method is accurate and reliable and can be applied to identify the four kinds of Gentianaceae Digeda-species mongolian medicine materials.