Objective: To set up an HPLC method for determination of morroniside and loganin in rat plasma

and study the pharmacokinetics of dispersible tablets of total glucosides from Cornus officinalis in rat plasma and tissues. Method: The procedure of HPLC was performed on a column of Diamonsil C18

taking methanol-water as mobile phase in gradient elution. The flow rate was 1 mL·min-1 and detection wavelength was 237 nm. The samples of plasma and tissues were prepared based on a simple protein precipitation with methanol. Result: The linear ranges of morroniside and loganin were

respectively

from 0.612 to 30.6 mg·L-1

and from 0.921 to 46.0 mg·L-1. The RSD values of intra-day and inter-day were less than 15%. The recovery was between 90.2% to 111.6%. After dispersible tablets of total glucosides from C. officinalis was administered orally

morroniside and loganin were found in plasma

stomach and small intestine samples. Conclusion: The method is simple and quick with a good repeatability

which can be used in the determination of plasma concentration of morroniside and loganin and pharmacokinetic study.