Objective: To establish the fingerprints of Woodwardiae unigemmmata from different habitats by HPLC. Method: HPLC was applied to detect W. unigemmmata samples from 9 different habitats with a DiamonsilTMC18 column by acetonitrile A-water B as mobile phase (gradient elute: 0-60 min

22%-100%A). The flow rate was 1.0 mL·min-1. The column temperature was at 25 ℃ and the UV detection wavelength was set at 265 nm. Result: The HPLC fingerprints of W. unigemmmata from different habitats were established and the correlation coefficients of them were calculated. Furthermore

two peaks in HPLC chromatogram were identified as kaempferol-3-O-α-L-rhamnopyranoside -7-O-α-L-rhamnopyranoside

kaempferol-3-O-α-L-(4-O-acetyl)-rhamnopyranoside-7-O-α-L-rhamnopyran-oside respectively. Conclusion: The method is simple

reproducible and can be used for quality control of W. unigemmmata.