HPLC测定丹黄祛瘀片中苦参碱和氧化苦参碱的含量

闵庆璐; 王巍; 鞠成国; 尹程鑫; 陈晓明

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HPLC测定丹黄祛瘀片中苦参碱和氧化苦参碱的含量

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- HPLC测定丹黄祛瘀片中苦参碱和氧化苦参碱的含量
- Determination of Contents of Matrine and Oxymatrine in Danhuangquyu Tablets by HPLC
- 中国实验方剂学杂志 2012年18卷第1期页码：60-62
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- 纸质出版日期：2012
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闵庆璐, 王巍, 鞠成国, 等. HPLC测定丹黄祛瘀片中苦参碱和氧化苦参碱的含量[J]. 中国实验方剂学杂志, 2012,18(1):60-62.

MIN Qing-lu, WANG Wei, JU Cheng-guo, et al. Determination of Contents of Matrine and Oxymatrine in Danhuangquyu Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 60-62.
闵庆璐, 王巍, 鞠成国, 等. HPLC测定丹黄祛瘀片中苦参碱和氧化苦参碱的含量[J]. 中国实验方剂学杂志, 2012,18(1):60-62. DOI：

MIN Qing-lu, WANG Wei, JU Cheng-guo, et al. Determination of Contents of Matrine and Oxymatrine in Danhuangquyu Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 60-62. DOI：

摘要

目的: 建立丹黄祛瘀片中苦参碱和氧化苦参碱的含量测定方法。方法: 采用HPLC

色谱柱为Agilent NH2柱(4.6 mm×250 mm

5 μm)

流动相为乙腈-无水乙醇-3%磷酸(82 ∶10 ∶8)

检测波长220 nm

柱温30 ℃

流速1.0 mL ·min-1。结果: 苦参碱和氧化苦参碱的线性范围分别为0.385~2.31(r=0.999 6)和0.021 8~0.239 8(r=0.999 2)μg;平均加样回收率分别为99.0% (RSD 0.85%

n=6)

97.2% (RSD 1.0%

n=6)。结论: 该法简便

快速

结果准确

重复性好

可用于控制丹黄祛瘀片的质量。

Abstract

Objective: To develop a HPLC method for the determination of matrine and oxymatrine in Danhuangquyu tablets. Method: An Agilent NH2 column (4.6 mm×250 mm

5 μm) was used

the mobile phase was acetonitrile-absolute ethyl alcohol-3% phosphoric acid (82 ∶10 ∶8)

the detection wavelength was 220 nm

the column temperature was 30 ℃ and the flow rate was 1.0 mL ·min-1. Result: Matrine showed a good linear relationship at the range of was 0.385-2.31 μg(r=0.999 6)

the average recovery was 99.0%

RSD 0.85% (n=6); Oxymatrine showed a good linear relationship at the range of was 0.021 8-0.239 8 μg(r=0.999 2)

the average recovery was 97.2%

RSD 1.0% (n=6). Conclusion: The method is accurate and reliable

and it can be used for the quality control of Danhuangquyu tablets.

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