Objective: To study protective effects and mechanism of serum containing Guipi decoction on bone marrow cells(BMCs) co-cultured with ethanol extraction of Tripterygium wilfordii in vitro. Method: BMCs were co-cultured in vitro with different concentrations of serum containing Guipi decoction and ethanol extraction of T. wilfordii. MTT assay was used to evaluate the growth proliferation of BMCs. The cell cycle and apoptosis rate were determined by flow cytometry(FCM) with propidium iodide(PI) staining and AnnexinⅤ/PI staining. Result: After treated with different concentrations of serum containing Guipi decoction

viability of BMCs was significantly increased. Cell cycle flow cytometry demonstrated that BMCs treated with different concentrations of serum containing Guipi decoction resulted in a decreased G0/G1 phase of BMCs and an increased S and G2/M phase and apoptosis rate was decreased. Conclusion: The different concentrations of serum containing Guipi decoction can significantly increase viability of BMCs

and significantly promote BMCs of G0/G1 phase entering proliferation cycle.