Objective: To study on prescription screening and preparation technology of genistein water-solubility suppository. Method: Optimum prescription of this water-solubility suppository with polyethylene glycol as main part was screened by orthogonal design test. Influence of different proportions of matrix on appearance and dissolution variable time limit of this suppository was investigated. Influence of molding temperature and cooling time on appearance and dissolution variable time limit of this suppository was investigated. Result: Range of influencing factors for genistein water-solubility suppository was S-40＞glycerine＞PEG 4000.The best prescription of genistein water-solubility suppository was PEG 4000-S-40-glycerol 6∶3∶4.Optimal molding temperature was 70 ℃

cooling time was 0.5 h. Conclusion: This optimized prescription was rational.Preparation technique was easy and practicable.