Objective: To establish the HPLC fingerprint of Faeces Trogopterori(FT). Method: The separation was performed on a Diamonsil ODS C18 column (4.6 mm×250 mm

5 μm ) and the mobile phase consisted of acetonitrile(A)-0.5% aqueous acetic acid (B)

with an optimized gradient program of 0~15 min

85%-65% B

15-35 min

65%-40% B

35-40 min

40%-25% B

40-60 min

25%-10% B

60-65 min

10%-0% B. The flow rate was 0.8 mL·min-1and the wavelength was set at 265 nm. The experiment was performed under room temperature. Result: The HPLC fingerprint of FT was established

in which 14 co-peaks were searched out and 3 peaks were identified with reference substances. All of the similarities were greater than 0.90.The precision

stability and repetition were good. Conclusion: The characteristic and specialization of the fingerprint were good

which can provide the basis for its quality control and effective substance.