Objective: To explore effects of total glucosides of peony(TGP) on experimental allergic encephalomyelitis (EAE) and the possible immunologic mechanisms. Method: Fifty female rats were randomly divided into five groups: the normal group

EAE model group and TGP low

medium

high dose

10 rats each group. TGP low

medium and high dose group were ig given 50

100

200 mg·kg-1·d-1

while the EAE model group was given equal dose of physiological salt water. The latent period and the maximal disease score of the rats and the level of Thl cytokines interferon gamma (IFN-γ )and tummor necrosis factor alpha (TNF-α) in blood serum were observed. Result: Each dose group of TGP had lighter symptoms than the EAE model group

the latent period was longer and the maximal disease score was lower than EAE model group. The level of TNF-α and IFN-γ in blood serum was lower than the EAE model group

which shoued a manner of dose-effect dependent. Conclusion: TGP has therapeutical effect on EAE

and the effect may be related to the decreased level of IFN-γ and TNF-α.