Objective: To investigate effects of the combination of Drynaria fortunei and Ploygonum multiflorum on alpha-glycosidase activity. Method: Following groups including enzyme

the combination of extracts of D.fortunei and P. multiflorum

acarbose and the positive drug

blank control and controls of the herbal extracts were established. The activity of different micro reaction systems like α-amylase or α-maltase

which were added to acarbose and the above correspoding groups

was determined by Bernfeld

and GOD. Result: The aqueous extract

sodden ointment of the combination of D.fortunei and P.multiflorum showed significant inhibition on α-maltase's activity with dose-effect relationship

consistent with the role of acarbose

but the inhibition was reversible and mixed. Conclusion: The aqueous extract and sodden ointment of D. fortunei and P.multiflorum combination have inhibition on α-glycosidase.