Objective: To establish conditions for content determination and entrapment efficiency determination of gentiopicroside lipsome. Method: Establish a HPLC method for determination of the content of gentiopicroside by methodology investigation;Separated gentiopicroside from gentiopicroside lipsome by centrifugation and equilibrium dialysis method

and determined respectively

calculated entrapment efficiency. Result: HPLC conditions for determination of gentiopicroside lipsome were:mobile phase of methanol-water(30: 70)

flow rate 1.0 mL·min-1

detection wavelength 270 nm;The average entrapment efficiency with centrifugation was 69.67%

and it was 76.12% with equilibrium dialysis method. Conclusion: This established HPLC conditions could used for determination of the content of gentiopicroside lipsome

determined entrapment efficiency with centrifugation was less than equilibrium dialysis.