Objective: In order to research at changes of several hydrolase activity of ginseng stems in the different growth stages of growing season. Method: With five-year old ginseng stems as material

neuter phosphoric acid buffer solution was adopted to extract thick liquid enzymes. UV spectrophotometry was applied to determine the activities of amylase (AMY)

esterase (EST)

acid phosphatase (ACP)

alkaline phosphatase (ALP) and phytase (PHY) in ginseng stems in the different growth periods. Result: Activity peak of AMY was appeared in the spreading stage and the flowering stage

and no significant changes were found in other growth stage. Activity peak of EST was appeared in the spreading stage

the flowering stage

the fruit growth stage

and the activity was reduced in the root growing after fruit stage. Activity peak of ACP and ALP were appeared in every stage in the whole growing season. Activity of PHY was relatively high in the fruit growth stage. Conclusion: Five kinds of hydrolase activity of ginseng stems have their own characteristic changes in different periods

which may be closely related to material metabolism

growth quality of ginseng.