Objective:To study effect of Shugan granule on proliferation and activation of rat hepatic stellate cells in vitro and investigate the main mechanism of Shugan granule in protection of liver fibrosis. Method: The experiment was divided into the blank control group

Shugan granule 0.01 g ·L-1 group

Shugan granule 0.02 g ·L-1 group and Shugan granule 0.04 g ·L-1 group.HSC was intervented in vitro for 24 h

then cell proliferation were found by MTT

the morphological changes were detected

α-smooth muscle actin(α-SMA) expression was investigated by Immunohistochemical method. Result: After 24 hours of incubation

Shugan granule at concentrations of 0.01

0.02

0.04 g ·L-1 reduced the rates of cell growth by 7.02%

13.33%

20.85%

respectively

showing inhibitory effects with concentration-dependent manner. Compared with the control group

cell proliferation and cell body extension was obviously inhibited by Shugan granule

the positive rate of expressed α-SMA in control group was(62.83±3.42)%

in Shugan granule 0.01 g ·L-1 group was(47.31±2.74)%

in Shugan granule 0.02 g ·L-1 group was(37.79±3.01)%

in Shugan granule 0.04 g ·L-1 group was(25.74±2.83)%

among control group and three Shugan granule intervention group there was a significant difference(P<0.05). Conclusion:Shugan granule can resist hepatic fibrosis through inhibiting the activation and prolifition of HSC.