Objective:To investigate optimization of preparation technology for paeonol cation liposome gel. Method:Paeonol cation liposome gel was prepared by dispersion-ultrasonic combined with grinding method

with encapsulation efficiency

particle size and ξ potential as indexes

L9(34)orthogonal design was used to optimize preparation technology

factors were chosen as amount of substance ratio of DC-Chol /Chol

lipid/drugs

hydration time and ultrasonic condition. Result:Optimum preparation technology was as follows:weigh of DC-Chol was 160 mg

Chol 300 mg and HSPC 500 mg

added into 40 mg paeonol solution dissolved by 15 mL chloroform

evaporated and removed organic solvent to form uniform film at 40 ℃

added 10 mL pH 6.5 phosphate buffer solution to hydrate for 2 h

probe ultrasound for 3min

and then filter by 0.8

0.45 μm microporous membrane

liposome solution was obtained. Meanwhile

took carbomer 1.0 g

added into liposome solution

swelled completely

dropped into 0.3 g glycerin

ground to form uniform gel preparations. Conclusion:Using film dispersion-ultrasonic and gridding method

we prepared cation liposomes gel containing paeonol with liposome having high entrapment efficiency

uniform size distribution and moderate ξ potential

gel preparation had moderate viscosity and slight irritation.