Objective: To optimize separation and purification technology of total flavones form Oroxylum indicum by macroporous resin

and establish optimum technology parameters. Method: Purification capacity of 5 different macroporous resins for total flavones form O. indicum with absorption ratio and desorption ratio as indexes by combining static and dynamic adsorption-desorption method. Result: HPD100 resin possessed good adsorption capacity and desorption rate by comparing with other resins.Optimized conditions of dynamic adsorption-desorption experiment were:concentration of sample was 4.618 g·L-1 (pH 2.0) at absorption flow speed of 3.0 mL·min-1

washed with 3 BV water and eluted with 5 BV of 90% ethanol. Conclusion: Under this optimized technology

HPD100 macroporous resin could be used to purify total flavones of O. indicum

the content of total flavones was more than 80%.