Objective: To observe the inhibition of different compatibility ratio of emblica total phenolic acids and flavonoids on tumors and the effects on the immune system. Method: 100 μL (containing 5 000 cells / mL of tumor cells) RPMI 1640 culture medium was added to each well of 96-well plate

the MTT method was used to observe inhibition of human liver cell lines HepG2 by different compatibility ratio of emblica total phenolic acids and flavonoids in vitro

64 mice were randomly divided into eight groups

namely normal control group and the total phenolic flavonoids emblica compatibility treatment group

oral administration was performed

once dayly

lasting 9 days. MTT was used to measure proliferation of mouse spleen lymphocyte transformation and determine the levels of serum interleukin(IL)-2 and interferon-gamma(IFN-γ) and immune function by different compatibility of emblica total phenolic acids and flavonoids ig 9 days. Result: The compatibility of different proportions of total phenolic flavonoids emblica significantly inhibited the liver tumor cells

when the ratio of flavonoidsand the total acid was 10∶1(110 mg ·L-1∶11 mg ·L-1) inhibiting rate was the highest. The compatibility of total Flavonoids(228 mg) and total phenolic acids (329.5 mg) promoted the proliferation of mouse spleen lymphocytes

and stimulation index was higher than the control group

promoting secretion of lymphocytes factors. Same results were found in the compatibility of total flavonoids (4.56 mg)and total phenolic acids (6.59 mg). Conclusion: Emblica total phenolic acids and flavonoids significantly can inhibite cell proliferation and enhance the bodys specific immune function and non-specific immune function