Objective: To optimize precipitation desalination technology for regeneration silk fibroin by orthogonal test. Method: Application of neutral calcium chloride prepared regenerated silk fibroin solution

desalination was applied by ethanol precipitation

desalination technology was optimized by orthogonal test with investigating factors(the concentration of ethanol

the amount of ethanol

precipitation time) of affecting precipitation process parameters for fibroin protein protein. Result: Optimum precipitation desalination technology for regeneration silk fibroin was:precipitated 4 h with 6 times the amount of 100%ethanol

at this point the concentration of silk fibroin solution was 0.08 g·mL-1. Precipitation desalination efficiency could reach 97.34%

yield of silk fibroin protein was 77.28%. Conclusion: This methord was stable and feasible

it had high desalination efficiency and could be used in desalination technology of protein.