HPLC测定齐酞酸钠含量及其有关物质

麻秀萍; 蒋朝晖; 贾宪生; 陈聪; 邓华湘

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HPLC测定齐酞酸钠含量及其有关物质

更新时间：2024-01-04

- HPLC测定齐酞酸钠含量及其有关物质
- HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances
- 中国实验方剂学杂志 2012年18卷第2期页码：89-91
- 作者机构：
- 作者简介：
- 基金信息：
  
  贵州省科技厅攻关项目(黔科合字[1998]9号)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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麻秀萍, 蒋朝晖, 贾宪生, 等. HPLC测定齐酞酸钠含量及其有关物质[J]. 中国实验方剂学杂志, 2012,18(2):89-91.

MA Xiu-ping, JIANG Zhao-hui, JIA Xian-sheng, et al. HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 89-91.
麻秀萍, 蒋朝晖, 贾宪生, 等. HPLC测定齐酞酸钠含量及其有关物质[J]. 中国实验方剂学杂志, 2012,18(2):89-91. DOI：

MA Xiu-ping, JIANG Zhao-hui, JIA Xian-sheng, et al. HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 89-91. DOI：

摘要

目的: 用高效液相色谱法测定齐酞酸钠的含量及其有关物质。方法: 采用 Eclipse XDB-C18色谱柱(4.6 mm×150 mm

5 μm)

以甲醇-乙腈-0.1%磷酸水溶液(80∶10∶10)为流动相

流速1.0 mL·min-1

柱温35 ℃

检测波长210 nm。结果: 齐酞酸钠与其相邻杂质峰能完全分离

齐酞酸钠在0.052 5~0.997 5 μg进样量与峰面积呈良好线性关系(r=0.999 9)

最低检出限为3 ng;精密度良好(RSD 0.38%)

平均回收率为99.53%(n=9)。结论: 该方法简便、准确、专属性好、灵敏度高

可用于齐酞酸钠的含量及有关物质测定。

Abstract

Objective: To determine the content and the related substances of oleanolic acid 3β-phthalic monoester disodium salt (OAPES) by HPLC. Method: The separation was performed on Eclipse XDB-C18 column(4.6 mm×150 mm

5 μm)

column temperature at 35 ℃ with mobile phase of methanol-acetonitrile-0.1% phosphoric acid solution(80∶10∶10). The flow rate was set at 1.0 mL·min-1 with detection at 210 nm. Result: There was a good linear relationship within the range of 0.052 5-0.997 5 μg(r=0.999 9)

the least detective range is 3 ng. The precision in a day is good (RSD 0.38%). The average recovery was 99.53%(n=9). Conclusion: The method is simple

accurate

specific and sensitiveness for the determination OAPES content and its related substances.

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