Objective: To investigate influence of sulfur fumigation on ecdysterone from Achyranthes bidentata. Method: The content changes of ecdysterone after fumigation of sulfur was determined by HPLC

XB-C18 column (4.6 mm×250 mm

5 μm)

mobile phase acetonitrile-water (15: 85)

detection wavelength 250 nm

column temperature 25 ℃

flow rate 0.8 mL·min-1. Result: Conditions of maximum amount of ecdysterone was:the amount of sulfur 100 g·m-3

fumigation once for 2 h;Linear relationship of ecdysterone in the range of 0.408-2.448 μg was good

the regression equation was Y=34.167X+61(r=0.999 6). Conclusion: Sulfur fumigation of ecdysterone from A. bidentata had a certain influence

the content of ecdysterone had a decreasing trends with increasing the amount of sulfur

prolonging exposure time and increasing fumigation times.