Objective: To investigate central ingredients of active components in Dachuanxiong fang (DF) by HPLC-DAD-MSn

so that the quality of DF as well as its preparations can be effectively and systemically controlled and the substance basis of DF can be explored. Method: HPLC fingerprint spectrum of the active parts of Dachuanxiong fang was established. HPLC-DAD-MSn was developed for identification of the active parts of Dachuanxiong fang. Result: Three components of the active parts of chuanxiong and 8 components of the active parts of tianma were identified. Conclusion: Research on central ingredients of active components in Dachuanxiong fang is favorable to the clarification of its active substance basis and perfection of quality stantard.