HU Yu-hua, DENG Shuang-bing, WANG Cheng-xia, et al. Study on Quality Standard of Shenghua Pills[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(7): 125-127.
HU Yu-hua, DENG Shuang-bing, WANG Cheng-xia, et al. Study on Quality Standard of Shenghua Pills[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(7): 125-127.DOI:
Objective: To establish a quality standard of Shenghua pills for scientific evaluation and effective control of its quality to provide a reliable basis. Method: TLC was used for identification; ferulic acid was determined by HPLC
the separation was performe on YMC-Pack ODS-A(4.6 mm×250 mm
5 μm)
using a mixture of methanol-1% acetic acid solution (25: 75) as the mobile phase with the flow rate of 1.0 mL·min-1
the detection wavelength is 322 nm. Result: Angelica sinensis (Oliv.)Diels.
Ligusticum chuanxiong Hort. and Glycyrrhiza uralensis Fisch. could be detected by TLC. Ferulic acid was linear in the range of 52.0-520.0 ng (r =0.999 7); the average recovery was 98.01% (RSD 1.34%). Conclusion: The method was simple and accurate with a good reproducibility
and can be used for the quality control of Shenghua pills.