Objective: To observe changes of mesenteric microcirculation in rats with syndrome of blood deficiency

based on micro image analysis. Method: Acetyl phenyl hydrazine(APH) was subcutaneously injected on the back of rats to establish a blood deficiency model. The blood flow of mesenteric microcirculation was observed based on BI-2000 medical image analysis system

and the microcirculation image was acquired and analyzed through LabVIEW IMAQ Vision software

and the blood velocity was measured by tracing leukocyte. Result: ①The color of mesenteric microcirculation in blood deficiency rats was dim

microvascular was deformed and its vascular wall was damaged. ②The change of blood velocity of mesenteric microcirculation was matched with findings of modern research. ③Blood flow of mesenteric microcirculation in blood deficiency rats showed a line flow state with particle and a aggregation flow state

and blood velocity was lower compared with common group(P<0.05). Conclusion: There exists vescular wall injury in rats with syndroms of blood deficiency

as well as blood stasis and exudation. Measurement of blood velocity by image ananlysis based on LabVIEW objectively can reflect pathological changes of mesenteric microcirculation in rats syndroms of with blood deficiency which shows that microscopic medical image analysis technique in terms of research traditional Chinese medicine syndrome of blood deficiency has a good application prospect.