WEN Ping, ZHAO Wen, YU Liang-zhong, et al. Quality Standard for Qianqu[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 77-79.
WEN Ping, ZHAO Wen, YU Liang-zhong, et al. Quality Standard for Qianqu[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 77-79.DOI:
Objective :To establish the quality standard for Qianqu. Method :Radix et Rhizoma Rhei
Radix aucklandiae and Fructus Aurantii immaturus were identified by TLC.Strychnine was determinated by HPLC
and chromatographic conditions was as follows: column of Dikma Diamonsil C18(4.0 mm×250 mm
5 μm)
mobile phase consisting of acetonitrile-0.02% H3PO4(19 ∶81)
flow rate of 1mL min-1
and detection wavelength of 283 nm. Result :The characteristic identification by TLC was distinct and highly specific. Strychnine showed a good linear relationship at a range of 10.568-528.4 mg·L-1(r=0.999 98)
the average recovery was 100.18%(RSD 2.73%)
precision with RSD of 0.37% (n=6)
repetition with RSD of 0.88% (n=6)and stability with RSD of 0.70%. Conclusion :The method is reliable
accurate
specific and can be used for quality control of the production.