Objective: Four kinds of Chinese medicinal materials (medicated leaven

Jianqu

pinellia rhizome fermented mass

semen sojae praeparatum) processed by fermentation method were studied through the correlative microbial culture

identification and analysis

to confirm the inherent quality and provide reference for using medicinal materials safely and effectively in clinical. Method: Four kinds of culture dishes (blood plate

Sabouraud

Chinese blue and conventional air) were used to make bacterial culture and morphological testing to the above Chinese medicinal materials. Result: The four Chinese medicinal materials were all cultured more miscellaneous bacteria in the conventional air dishes; gram-negative bacilli were cultured in some Chinese blue Petri dishes; while gram-positive bacillus

gram-negative bacillus

gram-positive coccus and fungal spores were cultured in blood plate in varying degrees; in sabouraud medium

only raw medicated leaven and raw Jianqu could culture fungoid which was distinguished as microzyme by morphological identification. Conclusion: Raw medicated leaven and Jianqu cultured more fungoid microzyme which were beneficial bacteria produced by fermentation. But fungoid have not been found in pinellia rhizome fermented mass and semen sojae praeparatum. Therefore

attention should be paid to the process of fermentation processing

so as to further ensure the quality of medicine materials.