Objective: To optimize ultrahigh pressure extraction (UPE) technology of salidroside from Rhodiola crenulata by central composite design/response surface methodology. Method: Central composite design was adopted for getting optimal ultrahigh pressure technique parameters

and compared with conventional extraction’s(reflux extraction

ultrasonic extraction

microwave extraction). Result: Optimal technology conditions of UPE for salidroside from R. crenulata were: solvent was 73.3% ethanol

ratio of solvent to material (mL:g) was 29.5

extraction pressure was 255.5 mPa

extracted 1 time with 2 min. Using this extracting process

extraction yield of salidroside was 9.29 mg ·g-1. It was 31.6% higher than reflux extraction

20.3% to ultrasonic extraction and 9.7% to microwave extraction. Conclusion: As a novel extraction technology of Chinese herbal medicine

UPE procedure had higher extraction yield

lower extracting temperature

required a shorter time and was less power consumption. It had provided a brand-novel method for extraction of salidroside from R. crenulata.