Objective: To establish a method for extracting

purifying and preparing isoguanosine from Croton tiglium. Method: Orthogonal design was used to optimize extraction technology parameters with the content of isoguanosine as index; Studying purification process of isoguanosine with macroporous resin and preparing high purity isoguanosine by preparative liquid chromatography. Result: Optimum extraction technology of isoguanosine was extracted 3 times with 8 times the amount of 20% ethanol

40 min each time. After preliminarily purifying by macroporous resin

isoguanosine was isolated from purified extracting solution and the purity was up to 98%. Conclusion: This method was simple and easy to operate

also could be used for preprartion high purity isoguanosine samples.