Objective:To observe effect of Jiawei Sini San(JWSNS) serum on proliferation of hepatic stellate cells(HSC-T6) and to investigate the mechanism of hepatic fibrosis. Method:Thirty rats were randomly divided into three groups: normal serum

colchicine group and Jiawei Sinisan group

10 rats each group. The normal serum group was given saline

10 mL ·kg-1 ig

once each day; the colchicine group was given colchicine

0.2 mg ·kg-1 ig

once each day; JWSNS group was given liquid of JWSNS 10 mL ·kg-1 ig

one each day. The three groups were administrated for 7 d. Drug serum was prepared with the medium to 5%

10%

20%

40%; hepatic stellate cells was cultured

logarithmic growth phase of cells

was assayed by MTT for measuring serum leptin stimulation of 100 ng ·mL-1 HSC-T6 proliferation. Result: JWSNS serum inhibited the proliferation of hepatic stellate cells (P<0.05 or P<0.01) with

a dose-dependent manner; colchicine control group also inhibited the proliferation of HSC-T6 cells (P<0.05)

but inhibition rate of 10% to 40%drug serum was significantly higher than that of colchicine group (P<0.05). Conclusion: JWSNS has the suppression on the proliferation of HSC-T6.