Objective :To establish an UPLC-MS method for the determination of medicinal ingrediants in Clindamycin Phosphate Glucose injection sterilized with high temperature. Method : LC condition: Adopted Acquity UPLC BEH C18column(2.1 mm×50 mm

1.7 μm

eluted gradiently with mobile phase consisted of acetonitrile and 0.02 mol·L-1 ammonium acetate buffer solution adjusted pH 3.0 with acetic acid)at a flow rate 0.25 mL·min-1.MS condition

adopted electrospray ionization(ESI)

and multiple reaction monitoring. Result :Clindamycin phosphate

clindamycin and lincamycin were detected in the clindamycin phosphate glucose injection sterilized with high temperature

and clindamycin is the maxest impurity. Conclusion :This method is selectivity and sensitive

it is suitable for detection of the medicinal ingrediants in clindamycin phosphate glucose injection sterilized with high temperature.