HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、 五味子甲素、五味子乙素和丹参酮Ⅱ的含量

秦建平; 吴建雄; 毕宇安; 林夏; 赵宾江; 王振中; 萧伟

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HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮Ⅱ的含量

更新时间：2024-01-04

- HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮Ⅱ的含量
- Determination of Schizandrin, Schisantherin A, Deoxyschizandrin, γ-Schishandrin and Tanshinone Ⅱ in Yixinshu Tablet by HPLC
- 中国实验方剂学杂志 2012年18卷第2期页码：77-80
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技部重大新药创制(2011ZX09401-097)
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  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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秦建平, 吴建雄, 毕宇安, 等. HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮Ⅱ的含量[J]. 中国实验方剂学杂志, 2012,18(2):77-80.

QIN Jian-ping, WU Jian-xiong, BI Yu-an, et al. Determination of Schizandrin, Schisantherin A, Deoxyschizandrin, γ-Schishandrin and Tanshinone Ⅱ in Yixinshu Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 77-80.
秦建平, 吴建雄, 毕宇安, 等. HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮Ⅱ的含量[J]. 中国实验方剂学杂志, 2012,18(2):77-80. DOI：

QIN Jian-ping, WU Jian-xiong, BI Yu-an, et al. Determination of Schizandrin, Schisantherin A, Deoxyschizandrin, γ-Schishandrin and Tanshinone Ⅱ in Yixinshu Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 77-80. DOI：

摘要

目的: 建立HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮ⅡA的含量。方法: 采用Phenomenex Kinetex C18柱

以乙腈-水梯度洗脱

流速1 mL·min-1

柱温30 ℃

检测波长250 nm。结果: 五味子醇甲在21.56~215.6 mg·L-1(r=0.999 9)

五味子酯甲在6.500~65.00 mg·L-1 (r=1)

五味子甲素在3.460~34.60 mg·L-1 (r=0.999 9)

五味子乙素在6.920~69.20 mg·L-1 (r=0.999 9)

丹参酮ⅡA在2.400~24.00 mg·L-1 (r=0.999 9)呈良好的线性关系

平均回收率五味子醇甲为97.92%

RSD 2.34%(n=6);五味子酯甲为100.28%

RSD 2.57%(n=6);五味子甲素为98.34%

RSD 2.73%(n=6);五味子乙素为98.06%

RSD 1.61%(n=6);丹参酮ⅡA为99.13%

RSD 1.42%(n=6); 结论: 本法简便、灵敏度高、重复性好

可用于益心舒片的质量控制。

Abstract

Objective: To establish a HPLC method for the determination of schizandrin

schisantherin A

deoyschizandrin

γ-schishandrin and tanshinone ⅡA in Yixinshu tablet. Method: The Phenomenex Kinetex C18 column was used with a mobile phase of acetonitrile-water by gradient elution

the flow rate was 1 mL·min-1

the column temperature was 30 ℃

the detection wavelength was at 250 nm. Result: The linear ranges of schizandrin

schisantherin A

deoxyschizandrin

γ-schishandrin and tanshinone ⅡA were 21.56-215.6 mg·L-1(r=0.999 9)

6.500-65.00 mg·L-1 (r=1)

3.460-34.60 mg·L-1 (r=0.999 9)

6.920-69.20 mg·L-1 (r=0.999 9) and 2.400-24.00 mg·L-1 (r=0.999 9) respectively.The average recoveries of five components were 97.92% with RSD 2.34%

100.28% with RSD 2.57%

98.34% with RSD 2.73%

98.06%with RSD 1.61% and 99.13% with RSD 1.42%. Conclusion: The method is simple

sensitive and repeatable.It can be a method for quality control of Yixinshu tablet.

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