Objective: To establish a simple determination method of polysaccharide in Rhizoma Polygonatii

which is not influenced by the content of monosaccharide. Method: The total sugar was determined by phenol-sulfuric acid method while the concentration of reducing sugar was determined by 3

5-dinitrosalicylic acid (DNS) method

the contentration of polysaccharide=Ct(the concentration of total sugar)-Cs(the concentration of reducing sugar). Result: It was used to evaluate the content of polysaccharide collected in different area

the concentrations of polysaccharide in Polygonatum Rhizoma collected fromYunnan province are relatively higher. Conclusion: The results showed above methods are convenient

stable and accurate

which can be used for quality evaluation of Polygonatum Rhizoma and its preparations.