Objective: To optimize separation and purification technology conditions of gentiopicroside

swertia bitter glycoside and swertia glycoside from Gentiana scabra. Method: The content of gentiopicroside

swertia bitter glycoside and swertia glycoside was determined by HPLC

optimum separation and purification process conditions of iridoid glycoside from G. scabra was screened by static and dynamic adsorption

desorption tests. Result: Optimum technology was:HPD300 macroporous resin was selected

the concentration of sample solution was 0.1 g·mL-1

adsoption flow rate was 1.0 BV·h-1

dosage of sample liquid was 10 BV

eluted by 2 BV water

then eluted with 8 BV 30% ethanol at eution flow speed was 2.0 BV·h-1. Conclusion: Gentiopicroside

swertia bitter glycoside and swertia glycoside from G. scabra were purified effectively with HPD300 macroporous resin

optimized process was simple

stable and feasble.