Objective: To establish callus culture method for Coptis deltoidea. Method: The possible effective factors of callus condition were studied by comparing with different medium

explants

temperature and light conditions; the response surface methodology (RSM) based on the Box-Behnken design of experiment was used to optimize the optimum hormone combination of callus proliferation. Result: The callus induction rate of stem tips was the highest about 12.67%. In the optimum culture conditions

the temperature was(15±2) ℃ and it was dark. The main factors affecting the callus proliferation of C. deltoidea were 2

4-D and KT

followed by NAA. Conclusion: The optimal medium for callus induction of C. deltoidea was 6

7-V + 2

4-D 5.0 mg ·L-1 + KT 2.0 mg ·L-1 + NAA 1.0 mg ·L-1. The optimum propagating medium was 6

7-V + 2

4-D 4.6 mg ·L-1 + KT 3.8 mg ·L-1 + NAA 2.4 mg ·L-1.