Objective: To optimize separation and purification technology of total alkaloids from Houttuynia cordata by macroporous resin. Method: With the content of berberine hydrochloride

pepper lactam and aristololactam B as indexes

separation and purification capacity of total alkaloids from H. cordata was compared with different types of macroporous resin and different technology conditions. Result: HPD-100 macroporous resin had the best separation and purification capacity for total alkaloids in following technology conditions: sample concentration was 20 g·L-1 with pH 3

flow rate was 0.5 mL·min-1

eluting reagent was ethanol solution of ammonia(ammonia concentration 0.5 mol·L-1

the volume fraction of ethanol 80%) with the amount of 4 BV

eluting velocity was 3.0 mL·min-1. Conclution: HPD-100 macroporous resin could be applied to purify alkaloids from H. cordata.