ZHANG Ming-yue, ZOU Yi-ke, WANG Cai-yun, et al. Optimum Extracting Method and Monosaccharide Composition of Polysaccharide from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 87-89.
ZHANG Ming-yue, ZOU Yi-ke, WANG Cai-yun, et al. Optimum Extracting Method and Monosaccharide Composition of Polysaccharide from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 87-89.DOI:
Objective:To optimize the extraction process of polysaccharide from Ligustrum lucidum Ait.(PLLA) and analyze monosaccharide compositionof PLLA. Method:With the extraction efficiency of polysaccharide as the index
an orthogonal design test was adopted to optimize the extraction process.The phenol-sulphuric acid colorimetric method was used to determine the content of PLLA. The PLLA was hydrolyzed by sulfuric acid
the hydrolyzate was assayed by HPLC. Result:The best extraction process of PLLA was added 10 times amount of water for 3 times and 3 hours for each time at the temperature of 100 ℃.PLLA composed of rhamnose
arabinose
mannose
glucose and their molratio is 3.71∶3.21∶1.00∶16.06. Conclusion: The method is simple
quick and accurate.This method could be applied for the analysis of monosaccharide composition and quality control in PLLA.