Objective: To explore the inhibiting the proliferation and the mechanism of human embro fibroblasts (MRC-5) by curcumol. Method: The effects of curcumol on the proliferation of MRC-5 were determined by MTT assay. The cotents of collagen accumulated were determined by the optical density at 540 nm of picro-sirius red staining. The cell cyclic retardation was examined by using flowcytometry. Result: MTT showed that curcumol(50-200 mg ·L-1) had an inhibitory effect on the proliferation and had reduced collagen of secretion in MRC-5. The better time in the 96 hour. The cell cycle analysia showed that curcumol(50-200 mg ·L-1) increased the percentage of G0/G1 phase cells

updown the percentage of S and G2/m phase cells. Conclusion :Curcumol can increase the percentage of G0/G1 phase cells

delay cell replication

inhibit the proliferation and collagen of secretion of MRC-5.