ZHAO Qi-peng, LAN Sha, ZHANG Yi. DPPH Radical Scavenging Effect of Compound Tangmaikang[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 41-45.
ZHAO Qi-peng, LAN Sha, ZHANG Yi. DPPH Radical Scavenging Effect of Compound Tangmaikang[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 41-45.DOI:
Objective: To establish a method of scavenging DPPH radical
and then evaluate DPPH radical scavenging effect of compound Tangmaikang. Method: Investigated the reaction time
temperature
the concentration of DPPH and the extraction solvent and method
the study on the DPPH radical scavenging effect of 14 batches Tangmaikang and drug-containing serum was carried out. Result: Tangmaikang was extracted with 60% methanol by ultrasonic for 30 min. The optimum reaction conditions were obtained as follows:DPPH concentration 0.099 8 g·L-1
reaction temperature 60 ℃
reaction time 50 min. The IC50 of 14 batches of Tangmaikang was 0.48 mg. The DPPH clearance of drug-containing serum was lower than blank serum. Conclusion: Tangmaikang has antioxidant activity in vitro.