Objective: To study on elution property of pigment and protein contained in polysaccharide from Chrysanthemum indicum. Method: Compared six different types of macroporous resin (LSA-700B

LSA-21

D101

XDA-8

AB-8

XDA-7) for purification effect of polysaccharide from C. indicum by selecting three indicators of decoloration rate

deproteinization rate and retained rate of polysaccharide; and investigated influence of temperature

polysaccharide concentration

pH

rotational speed and elution rate on purification property. Result: Optimum purification conditions of resin LSA-21 were as follows: temperature 40 ℃

polysaccharide concentration 7 g·L-1

pH 5

rotational speed 180 r·min-1 elution rate 3 BV·h-1

diameter/height ratio 1∶8.In these conditions

decoloration rate was up to 80.90%

deproteinization rate was 52.84% and retained rate of polysaccharide was 82.59%. Conclusion: High purification ratio and retained rate of polysaccharide could be obtained by means of purification with LSA-21 macroporus resin.