Objective: To determine the content of verbascoside in Callicarpa nudiflora. Method: HPLC was performed with chromatographic conditions as follows: Dikma Diamonsil C18 column(4.6 mm×250 mm

5 μm)

the mobile phase of acetonitrile-0.1% acetic acid (16 ∶84)

flow rate at 1 mL ·min-1

and the detection wavelength at 332 nm. Result: The linearity of verbascoside was good in the range 0f 8.24-206 mg ·L-1(r=0.999 5). The average recovery was 98.74%

RSD was 2.31%

with good repeatability RSD was 2.12%

(n=6) Conclusion: The method is simple

feasible and reproducible

and can be used to control the qualitv of C. nudiflora.