Objective: To study on optimum extraction technology for total flavonoids from fruits of Physalis alkekengi. Method: Based on single factor test

investigated influence of four factors to extract of total flavonoids from P. alkekengi by orthogonal design test.The four factors were concentration of ethanol、ratio of solid to liquid、extraction time and extraction times.Got optimal extraction technology by determined the content of total flavonoids from fruits of P. alkekengi UV-visible spectrophotometer. Result: The optimum extraction technology was as follows:extracted 3 times wtih 30 times amount of 60% ethanol for 30 minutes each times. The average extract of total flavonoids was 2.00 mg·g-1. Conclusion: Total flavonoids from fruits of P. alkekengi can be extracted with ethanol by ultrasonic wave.Ratio of liquid to solid was the most significant factor.