响应面法优化仙鹤草总黄酮的超声提取工艺

蒋志华; 俞力超; 冯子旺; 李峰; 徐秀泉

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响应面法优化仙鹤草总黄酮的超声提取工艺

更新时间：2024-01-04

- 响应面法优化仙鹤草总黄酮的超声提取工艺
- Optimization of Ultrasound Extraction Process of Total Flavonoids from by Response Surface Methodology
- 中国实验方剂学杂志 2011年17卷第20期页码：17-20
- 作者机构：
- 作者简介：
- 基金信息：
  
  镇江市社会发展项目(SH2010033)
- DOI：
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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蒋志华, 俞力超, 冯子旺, 等. 响应面法优化仙鹤草总黄酮的超声提取工艺[J]. 中国实验方剂学杂志, 2011,17(20):17-20.

JIANG Zhi-hua, YU Li-chao, FENG Zi-wang, et al. Optimization of Ultrasound Extraction Process of Total Flavonoids from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 17-20.
蒋志华, 俞力超, 冯子旺, 等. 响应面法优化仙鹤草总黄酮的超声提取工艺[J]. 中国实验方剂学杂志, 2011,17(20):17-20. DOI：

JIANG Zhi-hua, YU Li-chao, FENG Zi-wang, et al. Optimization of Ultrasound Extraction Process of Total Flavonoids from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 17-20. DOI：

摘要

目的: 优化仙鹤草总黄酮的最佳超声提取工艺。方法: 以总黄酮得率为指标

采用响应面设计方法对影响总黄酮得率的乙醇体积分数、超声温度及料液比进行优化。结果: 各因素对总黄酮提取率的影响大小依次为料液比-超声温度-乙醇体积分数。最佳工艺条件为72%乙醇

料液比1 ∶25

60 ℃下超声提取20 min。在此最佳条件下

总黄酮得率为9.02 mg·g-1

试验结果与模型预测值相符。结论: 利用超声提取仙鹤草总黄酮工艺稳定可靠。

Abstract

Objective: To optimize optimum ultrasound extraction process of total flavonoids from Agrimania pilosa. Method: With yield of total flavonoids as index

ethanol concentration

ultrasound temperature and material-liquid ratio were optimized by response surface methodology (RSM). Result: Effect factors on extraction rate of total flavonoids with the order of follows: material-liquid ratio

ultrasound temperature

ethanol concentration. The optimal extraction conditions were ethanol concentration was 72%

material-liquid ratio of 1 ∶25

extraction temperature was 60 ℃

extraction time was 20 min. Under these conditions

yield of total flavonoids reached 9.02 mg·g-1

which was agreed with model prediction. Conclusion: This extraction process of total flavonoids from A. pilosa by ultrasound-assisted was stable and reliable.

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