微波协助提取在中药饮片含量测定中的应用(3)——微波法与药典法测定土茯苓中落新妇苷含量比较

方婧; 付梅红; 杨洪军; 张贝贝; 王祝举; 杨岚; 杨雪燕; 张梦阳; 张康丁; 张东

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微波协助提取在中药饮片含量测定中的应用(3)——微波法与药典法测定土茯苓中落新妇苷含量比较

更新时间：2024-01-04

- 微波协助提取在中药饮片含量测定中的应用(3)——微波法与药典法测定土茯苓中落新妇苷含量比较
- Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (3) Comparison of Determination of Astilbin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method
- 中国实验方剂学杂志 2011年17卷第24期页码：43-45
- 作者机构：
- 作者简介：
- 基金信息：
  
  "重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划子课题(2006BAI09B07-02)
- DOI：
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- 纸质出版日期：2011
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方婧, 付梅红, 杨洪军, 等. 微波协助提取在中药饮片含量测定中的应用(3)——微波法与药典法测定土茯苓中落新妇苷含量比较[J]. 中国实验方剂学杂志, 2011,17(24):43-45.

FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (3) Comparison of Determination of Astilbin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 43-45.
方婧, 付梅红, 杨洪军, 等. 微波协助提取在中药饮片含量测定中的应用(3)——微波法与药典法测定土茯苓中落新妇苷含量比较[J]. 中国实验方剂学杂志, 2011,17(24):43-45. DOI：

FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (3) Comparison of Determination of Astilbin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 43-45. DOI：

摘要

目的: 建立微波协助提取法提取土茯苓中有效成分落新妇苷含量测定方法

比较微波协助提取法与药典法提取落新妇苷优势。方法: 采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm

5 μm)

流动相为甲醇-0.1%冰醋酸水溶液(39 ∶61)

检测波长291 nm

柱温30 ℃

流速1 mL·min-1。结果 :微波提取时间10 min

提取温度60 ℃

提取溶剂60%乙醇。落新妇苷在0.04~0.8 μg呈良好线性关系(r=0.999 9)。平均回收率99.80%(n=6)。结论 :微波提取法较药典法提取落新妇苷更简便快速、结果准确

为一种符合环保绿色理念的含量测定方法

可用于检测土茯苓饮片中有效成分落新妇苷含量。

Abstract

Objective :To establish a microwave-assisted extraction method for the extraction of the astilbin from Smilax glabra. Comparison of the determination of astilbin from Smilax glabra between microwave-assisted extraction and Chinese pharmacopeia method. Method :Dikma Diamonsil C18 column(4.6 mm×150 mm

5μm)was used. The mobile phase of methanol-0.1%acetic acid-water (39 ∶61). The temperature of column was 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 291 nm. Result :Microwave extractive time was 10 min

the temperature was 60 ℃

the extraction solvent was 60%ethanol. The calibration curve was in good linearity with in the range from 0.04 to 0.8 μg(r=0.999 9). The average recovery was 99.80% (n=6). Conclusion :The method is simple

rapid

as well as precise and reliable

and can be used for the determination of astilbin from Smilax glabra.

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