Objective :To improve the quality standard of Huoxiang Zhengqi Dripping pill. Method :On the original basis

the TLC identification of atractylodes lancea

tangerine peel

perilla leaf

liquoric root

pineuia ternata and determination the content of honokiol and hesperidin by HPLC were also revised. The HPLC separation was performed on Agilent ZORBAX SB-C18 column (4.6 mm×250 mm

5 μm)

the mixture of acetonitrile-0.1% acetic acid water was used as mobile phase in gradient elution. The detection wavelength was 294 nm. Result: The spot in the TLC was clear

and with no interference from negative control. In the HPLC experiments of precision

reproducibility and stability

the samples could be separated entirely. For hesperidin

honokiol and magnolol

the linear range was 0.199 1-3.982 0 μg(r=0.999 9)

0.135 4-2.707 0 μg(r=0.999 9)

0.107 2-2.144 0 μg (r=0.999 9) respectively

the average recoveries were 98.36% with RSD 0.86%;100.6% with RSD 1.04%; 98.11% with RSD 0.70% respectively. Conclusion :The method was sensitive simple

accurate and reproducible

can be used to control the quality of Huoxiang Zhengqi Dripping Pill better.