Objective :To explore the effects of different extracts from Cibotium barometz on proliferation of Osteoblasts.To compare the effects on proliferation of osteoblasts between crude and processed products

to explore the mechanism of anti-osteoporosis for C. barometz. Method :Osteoblasts with petroleum ether layer extract

chloroform layer extract

ethylacetate layer extract

butanol layer extract and polysaccharide from crude and processed products of C. barometz were cultured respectively. Meanwhile

after the rats were fed with each layer extract

the rat serum together with osteolastes was cultured. The proliferation was tested by MTT method. Result :In the different extract with osteoblasts test and the rat serum with osteoblasts test

the butanol layer extract had the most significant effect on cell proliferation of osteoblasts. And the effect of the processed product was more significant than that of crude product. Conclusion :The butanol layer extract from processed products of C. barometz had the most significant effect on cell proliferation of Osteoblasts.