Objective: The liposoluble constituents from Herba Gueldenstaedtiae Vernae were analyzed by GC-MS. Method: The liposoluble constituents were extracted by solvents petroleum ether and further identified by GC-MS and their relative content was calculated by area normalization method. Result: Through comparison with mass spectra database

twenty-two components

which accounted for 98.12% of the peak areas

were separated and identified. The major components were 3-(2-ethylhexyloxy)-propylamine (23.178%)

α-dihydrocaranine (20.388%)

2-methoxy benzenethiol (11.387%)

3-methylheptadecane (9.278%)

(Z)-3-hexadecene (7.000%). Conclusion: The liposoluble constituents have high application value in medicine and food industry and mainly consisted of alkane

olefin

ketone

phenol

alcohol

aldehyde

ester

etc. The present experiments provided a scientific basis for the further exploitation and utilization of this herb.