Objective: HPLC method was established for determination of ursolic acid and oleanolic acid in Radix Actinidiae. Method: HPLC was carried out on ZORBA×300SB-C18 (4.6 mm×250 mm

5 μm) at 25 ℃ using methanol-0.5% ammonium acetate (76 ∶24) as mobile phase with a flow rate of 1.0 mL ·min-1 and detection wavelength was at 210 nm. Result: Linear correlation (r = 0. 999 7) of the peak area and the concentration of ursolic acid over the concentration range 0.492-2.460 mg ·L-1 was obtained. Linear correlation (r = 0. 999 9) of the peak area and the concentration of oleanolic acid over the concentration range 0.448-2.240 mg ·L-1 was obtained. The average recovery was 97.6%

98.7% and RSD was 1.8%

0.6%. Conclusion: The method is simple

rapid and accurate. It can be used for the quality control of Radix Actinidiae.