加味四逆散对肝纤维化大鼠肝组织Ⅳ型胶原和 TGF mRNA表达的影响

郑旭锐; 李长秦; 孙守才; 宋健; 周滢

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加味四逆散对肝纤维化大鼠肝组织Ⅳ型胶原和 TGF mRNA表达的影响

更新时间：2024-01-04

- 加味四逆散对肝纤维化大鼠肝组织Ⅳ型胶原和 TGF mRNA表达的影响
- Effects of Jiawei Sini San on Expression of Collagen Ⅳ and Transforming Growth Factor-beta 1 of Hepatic Fibrosis in Rats
- 中国实验方剂学杂志 2011年17卷第21期页码：218-220
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- 纸质出版日期：2011
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郑旭锐, 李长秦, 孙守才, 等. 加味四逆散对肝纤维化大鼠肝组织Ⅳ型胶原和 TGF mRNA表达的影响[J]. 中国实验方剂学杂志, 2011,17(21):218-220.

ZHENG Xu-rui, LI Chang-qin, SUN Shou-cai, et al. Effects of Jiawei Sini San on Expression of Collagen Ⅳ and Transforming Growth Factor-beta 1 of Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 218-220.
郑旭锐, 李长秦, 孙守才, 等. 加味四逆散对肝纤维化大鼠肝组织Ⅳ型胶原和 TGF mRNA表达的影响[J]. 中国实验方剂学杂志, 2011,17(21):218-220. DOI：

ZHENG Xu-rui, LI Chang-qin, SUN Shou-cai, et al. Effects of Jiawei Sini San on Expression of Collagen Ⅳ and Transforming Growth Factor-beta 1 of Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 218-220. DOI：

摘要

目的: 观察加味四逆散对肝脏Ⅳ型胶原(C0L-Ⅳ)和转化生长因子β1(TGFβ1)mRNA的影响

探讨其抗肝纤维化的作用机制。方法: 将50只动物购回后随机分为4组

除空白对照组10只外

其余40只均用二甲基亚硝胺(DMN

用生理盐水稀释至5 g·L-1)以10 mL·kg-1体重ip

每日1次

每周连续3 d

连续4周的方法制备大鼠肝纤维化模型

加味四逆散按生药量以35 g·kg-1ig给药

采用实时定量PCR技术检测各组大鼠肝组织Ⅳ型胶原和TGFβ1 mRNA表达的水平。结果: 加味四逆散组大鼠肝组织C0L-ⅣmRNA为5.136±1.32

TGFβ1 mRNA为4.839±0.48

与病理模型组(C0L-ⅣmRNA 10.369±1.69

TGFβ1 mRNA 6.307±0.85)比较有显著性差异(P<0.05)。结论: 加味四逆散能够抑制肝纤维化大鼠肝组织C0L-Ⅳ和TGFβ1 mRNA表达

从而发挥其抗肝纤维化的作用。

Abstract

Objective: The mechanism of Jiawei Sini San(JWSNS) in treating hepatic fibrosis was rexplored and studied by observing the expression of collagen Ⅳ and transforming growth factor-beta (TGFβ1)mRNA in liver. Method: Fifty animals were randomly divided into 4 groups

in addition to 10 in the control group

the remaining 40 were treated with dimethylnitrosamine (DMN

diluted with saline to 5 g·L-1) to 10 mL·kg-1 ip

once daily for 3 d of each week

which lasted 4 weeks.The groups of treatment were fed with JWSNS gavage. Collagen Ⅳ and TGF-β1 were detected in liver samples by RT-PCR. Result: CoL-Ⅳand mRNA of Liver tissue in JWSNS group was 5.136 ± 1.32

TGFβ1 mRNA was 4.839±0.48

Compared with the pathological model group (CoL-Ⅳ mRNA 10.369±1.69

TGF β1 mRNA 6.307±0.85) the result were significantly different (P<0.05). Conclusion: The mechanism of treating hepatic fibrosis with JWSNS appears to be due to the enhancement of degradiation of Collagen Ⅳ and TGF-β1.JWSNS can treat hepatic fibrosis effectively.

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