Objective: To observe the effects of 6-gingerol on expression and transport ability of PepT1 in Caco-2 cells. Method: Caco-2 monolayers were grown on permeable supports. Peptide transport activity was studied using -glycyl-sarcosine ( -Gly-Sar). The densities of PepT1 protein and mRNA (SLC15A1) expression levels were analyzed by Western blot and Real-time quantitative polymerase chain reaction. Result: The total transported Gly-Sar of Caco-2 cells within 60

120 minutes of group treated by 6-gingerol were higher than those of controlled group

(6.84±0.46)

(8.61±0.54) μmol/per well vs (5.62±0.20)

(6.54±0.54) μmol/per well

and higher than group of cells treated together with Rp-8-Br-cAMP within 120 minutes

the latter result were (6.92±0.67) μmol/per well. And 6-gingerol showed up-regulated effect on PepT1 protein and mRNA expression

also can be inhabited by Rp-8-Br-cAMP. Conclusion: 6-gingerol has significant effects on promoting the transport ability of dipeptides in Caco-2 cells

which maybe take effects by up-regulating PepT1 protein and mRNA expression.