Objective: To establish the HPLC fingerprint chromatogram identification of the processed products from Gastrodia elata . Method: HPLC procedure was performed on a chromatographic column of Kromasil C18. Acetonitrile-glacial acetic acid water solution(0.5%)was used as mobile phase by gradient elution

and the detection wavelength was at 270 nm. The mobile phase flow rate was at 1.0 mL·min-1. The similarity and the clustering analysis were carried out by fingerprint chromatogram software. Result: The standard HPLC Fingerprint chromatogram was established through the determination of 10 batches processed products from 6 different producing areas. 15 peaks can be detected and the average similarity was over 98% in 10 samples. The processed products were almost the same on the whole chromatograms but the relative peak areas had the differences. The cluster analysis was consistent with the processed origins. Conclusion: HPLC method can be used in quality control of G. elata .