WU Yu-qiang, HOU Xiao-tao, GUO Zhen-wang, et al. Optimization of Extraction Technology for Polysaccharide from Sugarcane Leaves by Orthogonal Test with Multi-index[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 11-13.
WU Yu-qiang, HOU Xiao-tao, GUO Zhen-wang, et al. Optimization of Extraction Technology for Polysaccharide from Sugarcane Leaves by Orthogonal Test with Multi-index[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 11-13.DOI:
Objective: To optimize the extraction process of polysaccharide from sugarcane leaves by orthogonal design. Method: The yield of polysaccharide as index to optimize extraction process of polysaccharide from sugarcane leaves by orthogonal test with spectrophotometry.The content of polysaccharide was determined by phenol-sulfuric acid method. Result: The optimum extraction technology was as follows:extracted 3 times at 100 ℃ with 30 times amount of deionized water for 5 h per time. Conclusion: The verification test showed that the optimum extraction process was stable